Chicken non-histone chromosomal protein HMG-17 cDNA sequence
نویسندگان
چکیده
منابع مشابه
Single copy gene for the chicken non-histone chromosomal protein HMG-17.
A chicken genomic library was screened with the human cDNA encoding the non-histone chromosomal protein HMG-17 and a 4565-base pair fragment containing the entire gene encoding this protein was isolated and characterized. Sequence analysis of the fragment revealed that from the start to end of transcription, the HMG-17 gene is 3293 base pairs long and is comprised of 6 exons ranging in size fro...
متن کاملRecombinant human chromosomal proteins HMG-14 and HMG-17.
Vectors for expressing human chromosomal proteins HMG-14 and HMG-17 in bacterial cultures under the control of the temperature-inducible lambda PL promoter have been constructed. The open reading frames of the cDNAs have been amplified by the polymerase chain reaction (PCR), utilizing amplimers containing desired restriction sites, thereby facilitating precise location of the initiation codon d...
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A group of chromosomal non-histone proteins with affinity for DNA (NP) was isolated from rat liver and Novikoff hepatoma. This fraction, which represents less than 5% of the total chromatin protein content, binds preferentially to unique, double-stranded sequences of fractionated homologous DNA. The interactions are strong at low ionic strength (K, = 6.7 x lOme M) and decrease with rising salt ...
متن کاملThe primary structure of non-histone chromosomal protein HMG17 from chicken erythrocyte nuclei.
Chromatin contains a group of non-histone proteins called the high mobility group (HMG) proteins (reviewed [l]). There are 4 main HMG proteins in calf thymus, HMG 1,2, 14 and 17, which have all been shown to be present in isolated nucleosomes [2]. Some minor HMG protein components of calf thymus chromatin have also been characterised [3]. Attention has been focused on HMG14 and 17 because of th...
متن کاملA human placental cDNA clone that encodes nonhistone chromosomal protein HMG-1.
From a human placental lambda gt11 cDNA library, we have isolated a cDNA clone that encodes the entire 215-residue amino acid sequence of HMG-1. Analysis of an internal sequence similarity suggests that the DNA-binding domains of HMG-1 are separated by a rather long and flexible linker segment. Southern blotting of DNA digested with BamHI indicated a highly variable number of genes (or pseudoge...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1987
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/15.16.6750